Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation
oleh: Qiang Liu, Bing Wang, Takanori Katsube, Sai Jun Fan, Fei-Yue Fan, Hui Zhao, Xu Su, Jian Xiang Liu, Jia Cao, Li Qing Du, Chang Xu, Yan Wang
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2013-11-01 |
Deskripsi
Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.