CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis
oleh: GiWon Shin, Susan M. Grimes, HoJoon Lee, Billy T. Lau, Li C. Xia, Hanlee P. Ji
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-02-01 |
Deskripsi
Microsatellite genotyping is a common molecular method of for identification but current methods have restricted throughput. The authors demonstrate STR-Seq, which combines next generation sequencing with targetedin vitroCRISPR-Cas9 fragmentation to enable massively parallel analysis in the thousands.