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Isolation and Characterization of Microsatellite Loci for a Bioenergy Grass, <i>Miscanthus sacchariflorus</i> (Poaceae)
oleh: Hai-Fei Zhou, Shan-Shan Li, Song Ge
Format: | Article |
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Diterbitkan: | Wiley 2013-01-01 |
Deskripsi
Premise of the study: Microsatellite loci were developed for the biomass C4 grass, <i>Miscanthus sacchariflorus</i>, and proved to be suitable markers for population genetic studies and germplasm management of this species. Methods and Results: Twenty-three polymorphic microsatellite loci were identified from an enriched genomic library of <i>M. sacchariflorus</i>. The polymorphism was assessed in 50 individuals from two populations in China. The number of alleles per locus varied from two to 18, with a mean of 8.13. The observed and expected heterozygosities ranged from 0.2 to 1.0 and from 0.198 to 0.898, respectively. Conclusions: These new markers will be useful for further investigation of genetic diversity and population genetic structure as well as molecular breeding of <i>Miscanthus</i> species.