Generation of iPSC lines from primary human chorionic villi cells
oleh: Björn Lichtner, Peggy Matz, James Adjaye
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2015-11-01 |
Deskripsi
Primary human chorionic villi (CV) cells were used to generate the iPSC line by retroviral transduction of the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC. Pluripotency was confirmed both in vivo and in vitro. The transcriptomes of the CV-derived iPSC lines and the human embryonic stem cell lines—H1 and H9 have a Pearson correlation of 0.929 and 0.943 respectively.