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Detoxification of deoxynivalenol (DON) is one of the molecular mechanisms of resistance to Fusarium Head Blight desease in wheat, which causes by <em>Fusarium graminearum. </em>Mycotoxin producing Fusarium spices have some enzymes to reduce toxic effects. They can change trichothecenes to less toxic compounds by using trichothecene 3-O-acetyletransferase (<em>Tri101</em>) enzyme. This enzyme converts trichothecene to less toxic compounds by replaceing OH group on C3 with acetyl group. It had shown that a yeast acetyltransferase,<em> </em>AYT1<em>, </em>can convert DON to 3A DON and reduce it toxicity. In this study synthetic<em> AYT1</em> gene was used for tobacco transformation and transiently expressed in wheat plants. The results showed that the synthetic <em>AYT1</em> expresses in tobacco as well as wheat. Acetyltransferase activity analyses by thin layer chromatography confirmed that this enzyme can convert DON to 3-A DON that leads to reduce poisonous effect of mycotoxin.