Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage
oleh: Hong-Yan Zhao, Xia Huang, Ya-Ming Chai, Qian Ren, Xiang-Yu Ma, Hai Jiang, Ya-Fei Li, Dong-Ri Piao, Bu-Yun Cui, Jing Han, Hong-Yan Xiong, Cai-Zhong Zhu, Lan-Yu Li
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2009-07-01 |
Deskripsi
Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 ± 2 nm diameter) and short tails (32 ± 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage’s basic mechanisms and practical applications.