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This study investigated the benzimidazole resistance in <em>Haemonchus contortus</em> parasitic nematodes from sheep from October 2021 to December 2022 in Al-Diwanyiah city/Iraq, and samples were processed at the laboratory of Parasitology in Veterinary Medicine College of Al-Qadissyiah University through the detection of E198A mutation and SNP polymorphism of the β-tubulin gene at this codon position. Ninety adult <em>H. contortus</em> samples were collected from the abomasum of sheep (n = 400) and then tested by qPCR and tetra-primer ARMS-PCR. Of these, three different genotypes have been found for E198A SNP: heterozygous (RS), homozygous (SS), and homozygous resistant genotype (RR). The frequencies for these genotypes were 31.11% heterozygous, 57.77% homozygous, and 11.11% homozygous resistant. The current study indicated the spread of benzimidazole resistance for<em> H. contortus</em> of sheep in Iraq, Al-Diwnayiah by utilizing qPCR and tetra-primer ARMS-PCR for the first time. It is speculated that the BZ-resistance is due to excessive and irregular <em>H. contortus</em> drug abuse and inter-species transfer between ruminants at the commonly grazing pastures and from imported sheep.