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Changes of DNA Damage Effect of T-2 or Deoxynivalenol Toxins during Three Weeks Exposure in Common Carp (<i>Cyprinus carpio</i> L.) Revealed by LORD-Q PCR
oleh: Rubina Tünde Szabó, Mária Kovács-Weber, Krisztián Milán Balogh, Miklós Mézes, Balázs Kovács
Format: | Article |
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Diterbitkan: | MDPI AG 2021-08-01 |
Deskripsi
The present study aimed to adapt a Long-run Real-time DNA Damage Quantification (LORD-Q) qPCR-based method for the analysis of the mitochondrial genome of <i>Common carp</i> (<i>Cyprinus carpio</i> L.) and detect the DNA damaging effect of T-2 (4.11 mg kg<sup>−1</sup>) and deoxynivalenol (5.96 mg kg<sup>−1</sup>) mycotoxins in a 3-week feeding period. One-year-old <i>Common carp</i> were treated in groups (control, T-2 and DON). The mycotoxins were sprayed over the complete pelleted feed, and samples were taken weekly. Following the adaptation of LORD-Q PCR method for the <i>Common carp</i> species, the number of lesions were calculated to determine the amount of DNA damage. In the first and second weeks, the T-2 and the DON treated groups differed significantly from each other; however these differences disappeared in the third week. There was a significant difference in the DNA lesion values between weeks 1 and 3 in the deoxynivalenol-contaminated groups. While in the T-2 treated groups, the DNA lesion values were significantly reduced on weeks 2 and 3 compared to week 1. The results suggested that the trichothecene mycotoxins have a relevant DNA damaging effect.