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<strong>Background: </strong>This study aimed to analyze the effect of the Orientation Program based on Activities<br />of Daily Living on depression, anxiety, and stress in the elderly.<br /><strong>Methods: </strong>This is an experimental study on 65 elderly individuals living in 5 nursing homes in<br />Tehran in 2017 for 6 months (May to October). Samples were selected using non-random available<br />sampling methods and randomly assigned to intervention and control groups. Demographic<br />information questionnaire and 21-item version of the Depression Anxiety Stress Scale (DASS- 21)<br />were used. The elderly in the intervention group were subjected to the Orientation Program based on<br />Activities of Daily Living for 6 one-hour sessions for 1 month. DASS- 21 was measured before the<br />intervention, one month and two months after the intervention. Data were analyzed using SPSS V.<br />24 and Independent T-test, Mann-Whitney, Chi-Square and repeated measure ANOVA tests with a<br />significance level of P<0.05.<br /><strong>Results: </strong>The results showed differences between the two groups regarding depression, anxiety, and<br />stress one month after the intervention (P=0.003, P=0.005 and P<001, respectively). One month after<br />the intervention, the mean scores of depression, anxiety, and stress in the intervention group decreased<br />to 8±4.54, 4.5±3.09 and 7.53±5.42, respectively, and two months after the intervention, they decreased<br />to 8.25±4.44, 4.71±3.05 and 8.21±5.71, respectively.<br /><strong>Conclusion: </strong>According to the results, the Orientation Program based on Activities of Daily Living is<br />effective on depression, anxiety, and stress in the elderly. Considering the instability of the intervention<br />on the depression variable, further studies and long-term interventions are recommended.<br /><strong>Trial Registration Number: </strong>IRCT20180504039528N1