rpoB Gene Mutations in Rifampin-Resistant Mycobacterium tuberculosis Identified by Polymerase Chain Reaction Single-Stranded Conformational Polymorphism

oleh: Miriam Bobadilla-del-Valle, Alfredo Ponce-de-Leon, Catalina Arenas-Huertero, Gilberto Vargas-Alarcon, Midori Kato-Maeda, Peter M. Small, Patricia Couary, Guillermo M. Ruiz-Palacios, Jose Sifuentes-Osornio

Format: Article
Diterbitkan: Centers for Disease Control and Prevention 2001-12-01

Deskripsi

The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results. To determine the sensitivity of this method, we analyzed 35 RIFr strains and 11 rifampin-susceptible (RIFs) strains, using the DNA sequencing of the core region of rpoB for comparison. Of the RIFr, 24 had a PCR-SSCP pattern identical to that of H37Rv; the other 11 had four different patterns. The 11 RIFs had PCR-SSCP patterns identical to that of H37Rv. The sensitivity of the assay was 31.4%; its specificity was 100%. We observed a strong correlation between the degree of resistance and the type of mutation.