A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics
oleh: Jun Zhang, Wen Luo, Zhiyuan Wang, Xiaoyan Chen, Pengmei Lv, Jingliang Xu
| Format: | Article |
|---|---|
| Diterbitkan: | SpringerOpen 2021-08-01 |
Deskripsi
Highlights Co-cultivation was firstly carried out for the co-production of D-psicose and lipase. The carbon source glucose and fructose were fully utilized during co-culture. The yield of D- psicose was 11.70 g/L, and the conversion rate reached 69.54%. Self-induced expression of lipase was realized with enzyme activity of 16.03 U/mg. Metabolomics analyses were used to explore the co-culture effects of mixed strains.