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Plant NAC (NAM, ATAF1/2, and CUC2) family is involved in various development processes including Programmed Cell Death (PCD) associated development. However, the relationship between NAC family and PCD-associated cotton pigment gland development is largely unknown. In this study, we identified 150, 153 and 299 <i>NAC</i> genes in newly updated genome sequences of <i>G. arboreum</i>, <i>G. raimondii</i> and <i>G. hirsutum</i>, respectively. All <i>NAC</i> genes were divided into 8 groups by the phylogenetic analysis and most of them were conserved during cotton evolution. Using the vital regulator of gland formation <i>GhMYC2-like</i> as bait, expression correlation analysis screened out 6 <i>NAC</i> genes which were low-expressed in glandless cotton and high-expressed in glanded cotton. These 6 <i>NAC</i> genes acted downstream of <i>GhMYC2-like</i> and were induced by MeJA. Silencing <i>CGF1</i><i>(Cotton Gland Formation</i><i>1)</i>, another MYC-coding gene, caused almost glandless phenotype and down-regulated expression of <i>GhMYC2-like</i> and the <i>6 NAC</i> genes, indicating a MYC-NAC regulatory network in gland development. In addition, predicted regulatory mechanism showed that the <i>6</i> <i>NAC</i> genes were possibly regulated by light, various phytohormones and transcription factors as well as miRNAs. The interaction network and DNA binding sites of the 6 NAC transcription factors were also predicted. These results laid the foundation for further study of gland-related genes and gland development regulatory network.