Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species

oleh: Cintia V. Acuña, Pamela Villalba, H. Esteban Hopp, Susana N. Marcucci Poltri

Format: Article
Diterbitkan: Consejo Superior de Investigaciones Científicas (CSIC) 2014-12-01

Deskripsi

<p><em>Aim of study:  </em>To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different <em>Eucalyptus</em> species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cross-transferability of microsatellites markers (SSRs) located in candidate genes.</p><p><em>Area of study</em>: <em>Eucalyptus</em> species implanted in Argentina coming from different Australian origins.</p><p><em>Materials and methods: </em>Twelve validated and polymorphic SSRs in candidate genes (SSR-CGs) for wood quality in <em>E. globulus</em> were selected for cross species amplification in six species: <em>E. grandis, E. saligna, E. dunnii, E. viminalis, E. camaldulensis </em>and <em>E. tereticornis</em>.</p><p><em>Main results: </em>High cross-species transferability (92% to 100%) was found for the 12 polymorphic SSRs detected in <em>E. globulus.</em> These markers revealed allelic diversity in nine important candidate genes: cinnamoyl CoA reductase (CCR), cellulose synthase 3 (CesA3), the transcription factor LIM1, homocysteine S-methyltransferase (HMT), shikimate kinase (SK), xyloglucan endotransglycosylase 2 (XTH2), glutathione S-transferase (GST), glutamate decarboxylase (GAD) and peroxidase (PER).</p><p><em>Research highlights: </em>The<em> </em>markers described are potentially suitable for comparative QTL mapping, molecular marker assisted breeding (MAB) and for population genetic studies across different species within the subgenus <em>Symphyomyrtus</em>.</p><strong>Keywords</strong>: validation; cross-transferability; SSR; functional markers; eucalypts;<em> Symphyomyrtus.</em>