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Enterococci are ubiquitous in nature and normal commensals in the digestive tract, but they are one of the leading causes of nasocomial infections causing significant threat to public health because of the problem of antibiotic resistance. Majority of the isolates are Enterococcus faecalis and Enterococcus faecium but the incidence of other species shows an alarming rise (Prakash et al., 2005). Enterococcal spp. isolated from animals also showed considerable diversity (Jung et al., 2007). Enterococci can cause many economically important animal diseases including bovine mastitis and are considered as environmental pathogens as they are transmitted between the environment and the animal rather than from animal to animal (Rossitto et al., 2002). Enterococcal spp. are very often used as indicator bacteria for development of antimicrobial resistance and it provides accurate information on precise antibiotic treatment of the animals (CDC, 2002).