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Abstract High-resolution, dynamic assessments of the spatiotemporal distributions of populations are critical for urban planning and disaster management. Mobile phone big data have real-time collection, wide coverage, and high resolution advantages and can thus be used to characterize human activities and population distributions at fine spatiotemporal scales. Based on six days of mobile phone user-location signal (MPLS) data, we assessed the dynamic spatiotemporal distribution of the population of Xining City, Qinghai Province, China. The results show that strong temporal regularity exists in the daily activities of local residents. The spatiotemporal distribution of the local population showed a significant downtown-suburban attenuation pattern. Factors such as land use types, holidays, and seasons significantly affect the spatiotemporal patterns of the local population. By combining other spatiotemporal trajectory data, high-resolution and dynamic real-time population distribution evaluations based on mobile phone location signals could be better developed and improved for use in urban management and disaster assessment research.