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Abstract Background: Rate of injuries among female athletes is higher than male. Cyclic sex hormones fluctuation dur-ing the menstrual cycle might play an important role in this respect. The aim of this study was to investigate the rate of female athletes’ injuries in different phases of menstrual cycle. Methods: Athletes from twenty clubs participated in Iran league competition (2006-2007) were studied. Infor-mation of menstrual cycle and athletes' injuries was collected using injury report form completed by athletes and team physicians. Findings: The rate of injuries, ligament injury and also anterior cruciate ligament injuries were significantly higher in luteal phase, compare to ovulation and follicular phases (P < 0.01). When data were analysed based on the duration of each phase, more injuries were observed in the ovulation phase. Conclusion: There is circamenstrual variation in female athlete's sport injuries. So, for minimising rate of injury, female athletes should avoid from dangerous situations in training and competition during ovulation phase. Keywords: Injury, Menstrual cycle, Female athletes