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Turbulence parameters in the lower troposphere (up to ~4.5 km) are estimated from measurements of high-resolution and fast-response cold-wire temperature and Pitot tube velocity from sensors onboard DataHawk Unmanned Aerial Vehicles (UAVs) operated at the Shigaraki Middle and Upper atmosphere (MU) Observatory during two ShUREX (Shigaraki UAV Radar Experiment) campaigns in 2016 and 2017. The practical processing methods used for estimating turbulence kinetic energy dissipation rate <inline-formula> <math display="inline"> <semantics> <mi>&#949;</mi> </semantics> </math> </inline-formula> and temperature structure function parameter <inline-formula> <math display="inline"> <semantics> <mrow> <msubsup> <mi>C</mi> <mi>T</mi> <mn>2</mn> </msubsup> </mrow> </semantics> </math> </inline-formula> from one-dimensional wind and temperature frequency spectra are first described in detail. Both are based on the identification of inertial (&#8722;5/3) subranges in respective spectra. Using a formulation relating <inline-formula> <math display="inline"> <semantics> <mi>&#949;</mi> </semantics> </math> </inline-formula> and <inline-formula> <math display="inline"> <semantics> <mrow> <msubsup> <mi>C</mi> <mi>T</mi> <mn>2</mn> </msubsup> </mrow> </semantics> </math> </inline-formula> valid for Kolmogorov turbulence in steady state, the flux Richardson number <inline-formula> <math display="inline"> <semantics> <mrow> <msub> <mi>R</mi> <mi>f</mi> </msub> </mrow> </semantics> </math> </inline-formula> and the mixing efficiency <inline-formula> <math display="inline"> <semantics> <mrow> <msub> <mi>&#967;</mi> <mi>m</mi> </msub> </mrow> </semantics> </math> </inline-formula> are then estimated. The statistical analysis confirms the variability of <inline-formula> <math display="inline"> <semantics> <mrow> <msub> <mi>R</mi> <mi>f</mi> </msub> </mrow> </semantics> </math> </inline-formula> and <inline-formula> <math display="inline"> <semantics> <mrow> <msub> <mi>&#967;</mi> <mi>m</mi> </msub> </mrow> </semantics> </math> </inline-formula> around <inline-formula> <math display="inline"> <semantics> <mrow> <mo>~</mo> <mn>0.13</mn> <mo>&#8722;</mo> <mn>0.14</mn> </mrow> </semantics> </math> </inline-formula> and <inline-formula> <math display="inline"> <semantics> <mrow> <mo>~</mo> <mn>0.16</mn> <mo>&#8722;</mo> <mn>0.17</mn> </mrow> </semantics> </math> </inline-formula>, respectively, values close to the canonical values found from some earlier experimental and theoretical studies of both the atmosphere and the oceans. The relevance of the interpretation of the inertial subranges in terms of Kolmogorov turbulence is confirmed by assessing the consistency of additional parameters, the Ozmidov length scale <inline-formula> <math display="inline"> <semantics> <mrow> <msub> <mi>L</mi> <mi>O</mi> </msub> </mrow> </semantics> </math> </inline-formula>, the buoyancy Reynolds number <inline-formula> <math display="inline"> <semantics> <mrow> <mi>R</mi> <msub> <mi>e</mi> <mi>b</mi> </msub> </mrow> </semantics> </math> </inline-formula>, and the gradient Richardson number <i>Ri</i>. Finally, a case study is presented showing altitude differences between the peaks of <inline-formula> <math display="inline"> <semantics> <mrow> <msup> <mi>N</mi> <mn>2</mn> </msup> </mrow> </semantics> </math> </inline-formula>, <inline-formula> <math display="inline"> <semantics> <mrow> <msubsup> <mi>C</mi> <mi>T</mi> <mn>2</mn> </msubsup> </mrow> </semantics> </math> </inline-formula> and <inline-formula> <math display="inline"> <semantics> <mi>&#949;</mi> </semantics> </math> </inline-formula>, suggesting turbulent stirring at the margin of a stable temperature gradient sheet. The possible contribution of this sheet and layer structure on clear air radar backscattering mechanisms is examined.