Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2
oleh: Annabel K. Sangree, Audrey L. Griffith, Zsofia M. Szegletes, Priyanka Roy, Peter C. DeWeirdt, Mudra Hegde, Abby V. McGee, Ruth E. Hanna, John G. Doench
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-03-01 |
Deskripsi
Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here the authors make comparisons of numerous Cas9 variants, nominate options for base editing screens with denser coverage with A>G and C>T base editing screens and identify loss-of-function mutations in BRCA1 and Venetoclax-resistant mutations in BCL2.