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Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity
oleh: Yumeng Lin, Yang Chen, Qianhui Li, Xiwei Tian, Ju Chu
Format: | Article |
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Diterbitkan: | SpringerOpen 2019-05-01 |
Deskripsi
Abstract Background A rational and high-performance high-throughput screening system effectively improves the efficiency of strain screening. Results A rapid and efficient method was developed to detect carbon sources. And then by using glucose analog as screening pressure and two indexes for sophorolipids (SLs) production and glycerol consumption, a mutant of Candida bombicola L1.3 was successfully selected among 3000 mutants. It produces high SLs titer and co-utilizes glucose and glycerol simultaneously. Conclusions Compared to the wild-type strain, L1.3 exhibited 15.06% higher SLs titer and 35.69% higher glycerol consumption capacity in a 5-L bioreactor. We believe that L1.3 can potentially be used for the efficient industrial production of SLs with simple downstream processing for separating SLs and glycerol.