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The aim of this work was to assess the analgesic effect of three <i>Vitis vinifera</i> L. leaf extracts and the anti-inflammatory effect of three gels obtained from <i>Aesculus hippocastanum</i> L. seed extracts using animal models, as a preliminary study for the future development of topical preparations based on the combination of extracts with synergistic therapeutic effects on hemorrhoid disease. The analgesic effect was determined by means of the writhing test in mice. The anti-inflammatory effect was determined after administration of carrageenan or kaolin in the rat paw. Extraction using glycerol yielded the highest amounts of flavonoids for both <i>V. vinifera</i> leaves (37.27 &#177; 1.174 mg/L) and <i>A. hippocastanum</i> seeds (53.48 &#177; 0.212 mg/L). The highest total phenolic contents were registered for the <i>V. vinifera</i> 20% ethanolic extract (615.3 &#177; 34.44 mg/L) and for the <i>A.</i> <i>hippocastanum</i> glycerolic extract (247.8 &#177; 6.991 mg/L). The writhing test revealed that the <i>V. vinifera</i> ethanolic extract induced the most efficient analgesia (57.20%, <i>p</i> &lt; 0.01), better than that induced by the positive control. In the carrageenan inflammation model, only the gel obtained from the <i>A. hippocastanum</i> glycerolic extract significantly reduced paw edema (17.27%, <i>p</i> &lt; 0.05). An anti-inflammatory effect was also observed in the kaolin inflammation model but was not statistically significant (10.12%, <i>p</i> &gt; 0.05). Our findings indicate that <i>V. vinifera</i> and <i>A. hippocastanum</i> extracts may have potential uses for the treatment of pain and inflammation associated with hemorrhoid disease.