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Pine mushroom (<i>Tricholoma matsutake</i>) is one of the most valued ectomycorrhizal fungi in Asia because of its unique pine-like aroma; however, due to exceptionally slow growth of its mycelia in artificial conditions, its cultivation has been largely deemed as not possible. Previous studies have shown that some bacteria and a few <i>Trichoderma</i> species associated with pine mushroom promoted the growth of <i>T. matsutake</i> isolate, but this effect is relatively unexplored. In this study, we investigated the diversity of microfungi in the fairy ring of <i>T. matsutake</i> and their effect on the growth of <i>T. matsutake</i> isolate. From 184 fungal isolates, 28 species were identified based on suitable molecular markers. <i>Penicillium</i> was most frequently observed (16 species), followed by <i>Trichoderma</i> (4 species). Five Zygomycota species showed a high promoting effect on the growth of <i>T. matsutake</i> while the effects of ascomycetes were mixed. The microfungi that promote the growth of <i>T. matsutake</i> can be useful for forest nursery and artificial cultivation of <i>T. matsutake</i>.