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Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i>
oleh: Junyu Yao, Jiuyuan Bai, Sha Liu, Jingyan Fu, Ying Zhang, Tianshun Luo, Hongpei Ren, Rui Wang, Yun Zhao
Format: | Article |
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Diterbitkan: | MDPI AG 2022-12-01 |
Deskripsi
<i>Brassica napus</i> is a Cd hyperaccumulator, which is a serious threat to food and fodder safety. However, no related studies on developing Cd-safe <i>B. napus</i> have been reported yet. Here, we screened out a novel Cd uptake-related gene, <i>AtCUP1,</i> from the major facilitator superfamily in <i>Arabidopsis thaliana</i>. The mutation of <i>AtCUP1</i> decreased Cd accumulation, both in roots and shoots of <i>A. thaliana</i>. Furthermore, the disruption of the <i>AtCUP1</i> gene by the CRISPR/Cas9 system significantly reduced Cd accumulation in <i>A. thaliana</i>. Interestingly, the disruption of the <i>BnCUP1</i> gene, an orthologous gene of <i>AtCUP1</i>, by the CRISPR/Cas9 system also diminished Cd accumulation in both roots and shoots of <i>B. napus</i> based on the hydroponics assay. Furthermore, for the field experiment, the Cd accumulations of <i>BnCUP1</i>-edited lines were reduced by 52% in roots and 77% in shoots compared to that of wild-type (WT) lines, and the biomass and yield of <i>BnCUP1</i>-edited lines increased by 42% and 47% of that of WT, respectively. Noteworthily, agronomic characteristics of <i>B. napus</i> were not apparently affected by <i>BnCUP1</i>-editing. Thus, <i>BnCUP1</i>-edited lines are excellent non-transgenic germplasm resources for reducing Cd accumulation without a distinct compromise in yield, which could be applied to agricultural production in Cd-contaminated soils.