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Abstract Organic thin film transistors (OTFTs)‐based biosensors are widely applied as advanced biosensing platforms by virtue of their inherent ability to transfer and amplify received biological signals into electrical signals. Nevertheless, the development of OTFTs‐based biosensors with excellent sensitivity, selectivity, and stability for specific biological processes remains a major challenge. This mini review focuses on recent achievements in OTFTs‐based biosensors since 2010. Specifically, three types of OTFTs, specifically organic field‐effect transistors (OFETs), electrolyte‐gated OFETs (EGOFETs), and organic electrochemical transistors (OECTs) are summarized in terms of the key strategies required for high‐performance bioelectronics. Additionally, various OTFTs‐based biosensors, such as ions, glucose, nucleic acids, proteins, and cells are described in terms of their working principles. This mini review highlights the uses of OTFTs for a broad range of research applications with a focus on designing novel OTFTs‐based biosensors.