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<i>Leptosphaeriaceae</i> is a widely distributed fungal family with diverse lifestyles. The family includes several genera that can be distinguished by morphology and molecular phylogenetic analysis. During our investigation of saprobic fungi on grasslands in Yunnan Province, China, four fungal taxa belonging to <i>Leptosphaeriaceae</i> associated with grasses were collected. Morphological observations and phylogenetic analyses of the combined SSU, LSU, ITS, <i>tub</i>2, and <i>rpb</i>2 loci based on maximum likelihood and Bayesian inference were used to reveal the taxonomic placement of these fungal taxa. This study introduces four new taxa, viz. <i>Leptosphaeria yunnanensis</i>, <i>L. zhaotongensis</i>, <i>Paraleptosphaeria kunmingensis</i>, and <i>Plenodomus zhaotongensis</i>. Colour photo plates, full descriptions, and a phylogenetic tree to show the placement of the new taxa are provided.