Generation of induced pluripotent stem cell Line KAIMRCi001-A by reprogramming erythroid progenitors from peripheral blood of a healthy Saudi donor
oleh: Mohammad Al-Shehri, Moayad Baadhaim, Shereen Jamalalddin, Doaa Aboalola, Mustafa Daghestani, Hajar AlZahrani, Dalal Malibari, Mohammad Mubaraki, Kholoud Aldubayan, Mohammed AlBalwi, Khaled Alsayegh
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2021-10-01 |
Deskripsi
In this study we isolated and enriched erythroid progenitor cells (EPCs) from a 10 ml peripheral blood sample from a 37-year old healthy Saudi donor. After expansion, these EPCs were reprogrammed using episomal plasmids to generate an induced pluripotent stem (iPS) cell line, KAIMRCi001-A. The pluripotency of this line was confirmed by measuring the expression of typical pluripotency markers and assessing differentiation potential in vitro.