Real-time Analysis of Auxin Response, Cell Wall pH and Elongation in Arabidopsis thaliana Hypocotyls
oleh: Lanxin Li, S.F.Gabriel Krens, Matyas Fendrych, Jiri Friml
| Format: | Article |
|---|---|
| Diterbitkan: | Bio-protocol LLC 2018-01-01 |
Deskripsi
The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH.