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In the present study , Madagascar Periwinkle Catharanthus roseus (L.)G.Don alkaloids were evaluated against two cancer cell lines :larynx human cancer cell line Hep2,and mouse breast cancer cell line AMN3 , by using four  concentrations for each cell  line (25,12.5,6,25,3.125)mg/ml(in triplicate).and it has been found that alkaloids showed highest activity against larynx human cancer cell line Hep-2 ,while exhibited weak activity against mouse breast cancer cell line AMN3 compared with control treatment.