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This paper studied the pollution characteristics of shale oil refinery wastewater, by using conventional analysis, infrared spectroscopy, ultraviolet spectroscopy, chromatography and mass spectrometry. The results showed that: wastewater contains high concentration of dissolved organic matter, which was poor in biological treatment. The organic structure of wastewater contains groups such as benzene ring, carbonyl group, hydroxyl group, ether group and carboxyl group. The composition of wastewater was very complex, which has phenols and oil compounds, with inorganic pollutants such as iron, calcium, magnesium, and sodium.