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Living organisms live within a narrow zone of tolerance of climatic conditions. Fresh water fish Ophiocephalus unmourilius is found in Chanakapur dam of Girna river in Kalwan (M.S.). The fish showed irreversible changes in carbohydrates, proteins and Ascorbic acid content when exposed to cold stress. Due to cold stress glycogen and protein content of brain and muscles tissue show considerable increase where as liver tissue show decreasing trend. Similarly ascorbic acid content show increasing trend in brain but considerable decrease in liver and muscular tissue was observed.