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The research aimed to examine the effect of changes in spatial ammonia microclimatic due to zone placement and house length differences on the perfomance of broiler chicken at starter period. The material used were two units broilers closed house with different capacity, namely 60 x 12 m2 with 11,000 chickens and 120 x 12 m2 with 22,000 chickens, 600 DOC strain Cobb were allocated in a completely randomized block design of split-plot with two main plots, those were house length 60 m and 120 m, and four subplot were placement zone of broiler chicken consists of Zone 1 (parallel to the inlet), Zone 2 (¼ house length), Zone 3 (½ house length), Zone 4 (¾ house length). The parameter observed were body weight, average daily gain, feed conversion ratio, index perfomance, Income Over Feed Cost (IOFC), macroclimate and microclimatic in each unit of chicken placement zone and house length differences as an illustration of the research condition. The results showed that there were interactions between the placement zone and house length on broiler performace. The further placement zone and the longer house distance from the inlet significantly (P<0.05) reduced the body weight, average daily gain, index perfomance and IOFC, while increased food conversion ratio. The conclusions of this study was the spatial microclimatic increase of ammonia in the placement zone of chickens which was farther from the inlet and the longes cages decreases the broiler performance at starter period.