A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy
oleh: Wenjing Yin, Derrick Brittain, Jay Borseth, Marie E. Scott, Derric Williams, Jedediah Perkins, Christopher S. Own, Matthew Murfitt, Russel M. Torres, Daniel Kapner, Gayathri Mahalingam, Adam Bleckert, Daniel Castelli, David Reid, Wei-Chung Allen Lee, Brett J. Graham, Marc Takeno, Daniel J. Bumbarger, Colin Farrell, R. Clay Reid, Nuno Macarico da Costa
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-10-01 |
Deskripsi
Electron microscopy (EM) is the gold standard for biological ultrastructure but acquisition speed is slow, making it unsuitable for large volumes. Here the authors present a parallel imaging pipeline for continuous autonomous imaging with six transmission EMs to image 1 mm3 of mouse cortex in less than 6 months.