The novel gene VpPR4-1 from Vitis pseudoreticulata increases powdery mildew resistance in transgenic Vitis vinifera L.
oleh: Lingmin eDai, Lingmin eDai, Lingmin eDai, Dan eWang, Dan eWang, Dan eWang, Xiaoqing eXie, Xiaoqing eXie, Xiaoqing eXie, Chaohong eZhang, Chaohong eZhang, Chaohong eZhang, Xiping eWang, Xiping eWang, Xiping eWang, Yan eXu, Yan eXu, Yan eXu, Yuejin eWang, Yuejin eWang, Yuejin eWang, Jianxia eZhang, Jianxia eZhang, Jianxia eZhang
| Format: | Article |
|---|---|
| Diterbitkan: | Frontiers Media S.A. 2016-05-01 |
Deskripsi
Pathogenesis-related proteins (PRs) can lead to increased resistance of the whole plant to pathogen attack. Here, we isolate and characterize a PR-4 protein from a wild Chinese grape Vitis pseudoreticulata which shows greatly elevated transcription following powdery mildew infection. Its expression profiles under a number of abiotic stresses were also investigated. The PR-4 gene was overexpressed in regenerated V. vinifera cv. Red Globe via Agrobacterium tumefaciens-mediated transformation and verified by the Western blot. The transgenic grapevines exhibited higher expression levels of PR-4 protein content than wild-type vines and also repressed the growth of powdery mildew. The PR gene responds differently to different stresses in the PR-4 transformants. This study demonstrates that PR-4 protein in grapes plays a vital role in defense against powdery mildew invasion.