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In this article, the investigation of electrochemical determination of guanine(GU) by 1-phenyl-3-methyl-4-(2-furoyl)-5-pyrazolone (HPMαFP) modified glassy carbon electrode (HPMαFP/GCE) in Aciclovir Dispersible Tablets solution was reported. The direct electrochemical behavior of GU was carefully studied in pH 5.5 phosphate buffer solution. Cyclic voltammogram results indicated that a well-defined irreversible redox peak was obtained with the formal potential of 0.92 V. The electrochemical parameter such as the electron transfer number (n) was calculated as 2. The HPMαFP modified electrode showed good electrocatalytic ability to GU. The concentration linear equation is as follows: iP (μA)=4.3767C (μmol l-1)+9.51332 with the detection limit of 1.2×10-7 mol/l.