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We sequenced almost complete mitochondrial genome of Apis florea (Insecta: Hymenoptera: Apocrita: Apidae) with length of 15,933 bp. The genome has similar codon usage and gene organization to those of mitogenome reported for other Hymenoptera. It includes 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and a noncoding region with very high AT-base content. Phylogenetic analyses showed that, Apis florea and (Apis cerana+ Apis mellifera) are the sister taxa, and more original, which is widely accepted view.