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Ozone is a robust antimicrobial agent with numerous potential applications in the industry. Ozone, in either gaseous or aqueous phases is effective against the majority of microorganisms. Relatively low concentrations of ozone and short contact time are sufficient to inactivate bacteria and microorganism. This project investigates the efficacy of dissolved ozone against food-related microorganism. The ozone system is evaluated using the microbial effects on Staphylococcus aureus, and Bacillus cereus and determine its clinical efficacy against ORP level for disinfection application. Results showed that 100% of S. aureus and B. cereus were eliminated by the dissolved ozone in tap water. In conclusions, the dissolved ozone has great efficacy, lower cost and less time disinfection cycle. Thus, this low temperature, ozone-based disinfection is a green technique and is regarded as one of the most promising disinfection techniques.