Find in Library

Rhizopus oryzae is classified a one of the Mucorales which belong to the zygomycota. This microorganism is a filamentous fungus can produce various substances such as ethanol, fumaric acid, lactic acid, ragi, and temph. R. oryzae has been occupied important role in the producing benefit metabolites such as organic acids. Enzymes. and antimicrobial compounds. In the current study, potato dextrose agar (PDA) and malt extract agar (MEA) were used for growing R. oryzae that the fungus was identified depending on the morphological parameters and conventional PCR. The fungus was cultured in two synthetic media were corn steep liquor (SCL) and conocarpus leaves (CL) at 25°C. R. oryzae produced abundant growth in both media and the growth was greyish white. GC-MA analysis appeared several different compounds in the crude extracts of R. oryzae using these fermenting synthetic media; however, some compounds were reported to be similar in their structures. The similar compounds were (pyrrolo [1,2-a] pyrazine-1,4-dione, hexahydro-), (1-nonadecene), (pyrrolo [1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl),) methyl stearate), (hexanedioic acid, bis(2-ethylhexyl) ester), (bis(2-ethylhexyl) phthalate), and (1,4-benzenedicarboxylic acid, (bis (2-ethylhexyl) ester). This study aimed to evaluate CL medium in comparison with CSL one for detecting the natural compounds produced from R. oryzae.