In vitro Autophosphorylation and Phosphotransfer Assay of Cyanobacterial Histidine Kinase 2

oleh: Iskander M. Ibrahim

Format: Article
Diterbitkan: Bio-protocol LLC 2016-12-01

Deskripsi

This is a detailed protocol of an autophosphorylation and phosphotransfer activities of Synechocystis sp. PCC 6803 full-length Histidine Kinase 2 (Hik2) protein described by Ibrahim et al., 2016. In this protocol, radioactively labelled ATP was used to study an autophosphorylation and phosphotransfer activity of the full-length Hik2 protein.