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In vitro Autophosphorylation and Phosphotransfer Assay of Cyanobacterial Histidine Kinase 2
oleh: Iskander M. Ibrahim
Format: | Article |
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Diterbitkan: | Bio-protocol LLC 2016-12-01 |
Deskripsi
This is a detailed protocol of an autophosphorylation and phosphotransfer activities of Synechocystis sp. PCC 6803 full-length Histidine Kinase 2 (Hik2) protein described by Ibrahim et al., 2016. In this protocol, radioactively labelled ATP was used to study an autophosphorylation and phosphotransfer activity of the full-length Hik2 protein.