Whole-brain tissue mapping toolkit using large-scale highly multiplexed immunofluorescence imaging and deep neural networks
oleh: Dragan Maric, Jahandar Jahanipour, Xiaoyang Rebecca Li, Aditi Singh, Aryan Mobiny, Hien Van Nguyen, Andrea Sedlock, Kedar Grama, Badrinath Roysam
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-03-01 |
Deskripsi
It is challenging to map complex processes in brain tissue. Here the authors report a toolkit enabling large-scale multiplexed IHC and automated cell classification whereby they use a conventional epifluorescence microscope and deep neural networks to phenotype all major cell classes of the brain.