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Objectives: The present study aimed to investigate the relationship between religious coping and quality of life of mothers of children with hearing deficiency.  Methods: In this study, 55 mothers of children with hearing deficiency referred to Isfahan Cochlear Implant Center, from June to August 2012, were selected through applying census method. The participants answered the questionnaires of Religious Coping and Quality of Life (FS36). Results: Results of correlation analysis indicated that there was a significant negative relationship between negative emotions toward God and the mental dimension of quality of life. Moreover, there was a significant positive relationship between benevolent appraisal and the physical dimension of quality of life. Regression analysis showed that negative emotions could explain 7.2% of variations in the mental dimension of respondents’ quality of life. Discussion: The increase in negative religious coping behaviors (e.g. negative emotions toward God) was associated with reduction of quality of life of mothers of children with hearing deficiency.