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AIM: To evaluate the clinical feasibility of using a new optical coherence interferometry(IOL-Master), comparing with traditional ultrasonic biometry and manual keratometry in the accuracy and characteristics for intraocular lens calculation of high myopia.<p>METHODS: The measurement of axial length was performed in 60 eyes(30 eyes for each group)with senile cataract of high myopia(≥-6.00D)using IOL-Master and ultrasonic biometry. The measurement of corneal power(K)was also performed in the patient using IOL-Master and manual keratometry preoperatively. Phacoemulsification and foldable lens implantation were done on the patients. IOL power calculation was carried out according to the SRK/T formula on the basis of the group-related data. Best corrected visual acuity, refraction, contrast sensitivity and wave front aberration root mean square(RMS)were re-tested after 3 months postoperatively.<p>RESULTS: Significant difference between the two methods in axial length measurement which was 29.81±1.53mm by ultrasound and 29.63±1.81mm by IOL-Master(<i>P</i>=0.001). And in corneal power measurement which was 43.22±1.67K by manual keratometry and 44.27±1.39K by IOL-Master(<i>P</i>=0.006). There was a significant difference between the two groups(<i>P</i>=0.001). 63.0% <i>vs</i> 31.2% had a mean absolute refractive error(MARE)within ±0.50 diopter for the IOL-Master and A-scan groups, respectively(<i>χ</i>²=3.1, <i>P</i><0.05). The RMS values of 4^th order aberration, 4^th order spherical aberration and total high order aberration in the IOL-Master group were lower than those in the A-scan group at 6mm pupil diameter 3 months later. <p>CONCLUSION: IOL-Master is a non-contact, accurate, safe and reliable tool for calculating IOL power and it is more accurate on the design of the IOL in the cataract surgery on the high myopia patients.