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This study evaluated the role of carotenoids in regulate the skin color of Kissing Gourami (Helostoma temminckii). 40 fishes were randomly selected into 2 groups (20 each group). Control group was fed with standard flake feed. Astaxanthin (12mg/kg) and β-carotene (157 mg/kg) were added to the standard diet for experimental group. Fish skin colour was compare with colour scale. Colour change was recorded weekly for the 12 weeks of experiment. First colour change was record in 5 th week in control group. No other change of colour was determinate. In experimental group was first colour change in 3rd week and the maximal influence of carotenoids was recorded in 8th week. No other colour change was record in experimental group to the end of experiment.