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The present work shows the obtainment of biosynthesized SiO₂ with the aid of <i>Jasminum grandiflorum</i> plant extract and the study of its photocatalytic ability in dye degradation and antibacterial activity. The obtained biosynthesized SiO₂ nanoparticles were characterized using X-ray diffractometer analysis, Fourier transform infrared spectroscopy analysis, ultraviolet–visible diffuse reflectance spectroscopy, field-emission scanning electron microscope with energy-dispersive X-ray analysis, transmission electron microscopy and X-ray photoelectron spectroscopy. The UV-light irradiated photocatalytic activity of the biosynthesized SiO₂ nanoparticles was examined using methylene blue dye solution. Its reusability efficiency was determined over 20 cycles and compared with the commercial P-25 titanium dioxide. The bacterial resistivity of the biosynthesized SiO₂ nanoparticles was examined using <i>S. aureus</i> and <i>E. coli.</i> The biosynthesized SiO₂ nanoparticles showed a high level of crystallinity with no impurities, and they had an optimum crystallite size of 23 nm, a bandgap of 4 eV, no Si-OH groups and quasi-spherical shapes with Si-2p at 104 eV and O-1s at 533 eV. Their photocatalytic activity on methylene blue dye solution could reach 90% degradation after 40 min of UV light exposure, and their reusability efficiency was only 4% less than that of commercial P-25 titanium dioxide. At the concentration of 100 μg/mL, the biosynthesized SiO₂ nanoparticles could allow the resistivity of <i>E. coli</i> to become borderline to the resistant range of an antibiotic called Amikacin.