Directed evolution of CRISPR-Cas9 to increase its specificity
oleh: Jungjoon K. Lee, Euihwan Jeong, Joonsun Lee, Minhee Jung, Eunji Shin, Young-hoon Kim, Kangin Lee, Inyoung Jung, Daesik Kim, Seokjoong Kim, Jin-Soo Kim
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-08-01 |
Deskripsi
Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.