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Objective: This work aimed to determine the resistance and/or the susceptibility to antibiotics of staphylococci isolated from cattle with mastitis in the North of Algeria. Materials and Methods: The disk diffusion method was carried out to reveal the antibiotic resis¬tance in accordance to the National Committee for Clinical Laboratory Standards guidelines in the Mueller-Hinton agar. Results: Coagulase-negative Staphylococci (CNS) isolates showed more resistance to Cefoxitin, Amoxicillin + Clavulanic Acid, Vancomycin, Trimethoprime Sulfamethoxazole, Clindamycine, Neomycin, and Erythromycin than Coagulase-positive Staphylococci (CPS). CPS were more resis¬tant to Penicillin and Tetracycline as compared to CNS strains; however, all these strains presented sensitivity to Gentamicin and neomycin. Conclusion: The Staphylococci showed high resistance to the beta-lactam antibiotics. As far as the authors know, these molecules are used with or without control in different protocols to prevent and cure the mastitis in Algeria. [J Adv Vet Anim Res 2019; 6(2.000): 231-235]