Alternative <i>c-MYC</i> mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 Tumors

oleh: Dina Ibrahim, Léa Prévaud, Nathalie Faumont, Danielle Troutaud, Jean Feuillard, Mona Diab-Assaf, Ahmad Oulmouden

Format: Article
Diterbitkan: MDPI AG 2022-06-01

Deskripsi

While studying c-Myc protein expression in several Burkitt lymphoma cell lines and in lymph nodes from a mouse model bearing a translocated <i>c-MYC</i> gene from the human BL line IARC-BL60, we surprisingly discovered a complex electrophoretic profile. Indeed, the BL60 cell line carrying the <i>t</i>(8;22) <i>c-MYC</i> translocation exhibits a simple pattern, with a single c-Myc2 isoform. Analysis of the <i>c-MYC</i> transcripts expressed by tumor lymph nodes in the mouse <i>λc-MYC</i> (<i>A<sup>vy</sup>/a</i>) showed for the first time five transcripts that are associated with <i>t</i>(8;22) <i>c-MYC</i> translocation. The five transcripts were correlated with the production of c-Myc2 and c-MycS, and loss of c-Myc1. The contribution of these transcripts to the oncogenic activation of the <i>t</i>(8;22) <i>c-MYC</i> is discussed.