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<em><span lang="EN-US">The anodic oxidation of <span class="SpellE">oxytetracycline</span> was performed with success using as anode a boron-doped diamond electrode. The experiments were conducted in batch mode, using two different electrochemical cells: an up-flow cell, with recirculation, that was used to evaluate the influence of recirculation flow rate; and a stirred cell, used to determine the influence of the applied current density. Besides <span class="SpellE">oxytetracyclin</span> <span class="SpellE">electrodegradation</span> rate and mineralization extent, oxidation by-products were also assessed. Both the flow rate and the applied current density have shown positive influence on the <span class="SpellE">oxytetracycline</span> oxidation rate. On the other hand, the mineralization degree presented the highest values at the lowest flow rate and the lowest current density tested. The main oxidation by-products detected were oxalic, <span class="SpellE">oxamic</span> and maleic acids.</span></em>