Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization
oleh: Serif Senturk, Nitin H. Shirole, Dawid G. Nowak, Vincenzo Corbo, Debjani Pal, Alexander Vaughan, David A. Tuveson, Lloyd C. Trotman, Justin B. Kinney, Raffaella Sordella
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-02-01 |
Deskripsi
CRISPR/Cas9-based genome editing enables specific deletion of target genes. Here, Senturket al. develop an inducible editing system by fusing a conditional destabilization domain to Cas9 and demonstrate temporal control of gene editing in response to a synthetic ligand.