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The plant defense hormone jasmonates not only play important roles in plant growth, development, and resistance, but also hold promise for bringing new strategies in plant protection and cancer therapy. Recently, de novo biosynthesis of natural and unnatural jasmonates in refactored yeast with integration of 15 heterologous genes and 3 native genes deleted was reported. Here, we highlight the feasible and sustainable platform to efficiently produce jasmonates, which would benefit both agriculture and human health.