Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
oleh: Jesse J. Waggoner, Victoria Stittleburg, Renee Pond, Youssef Saklawi, Malaya K. Sahoo, Ahmed Babiker, Laila Hussaini, Colleen S. Kraft, Benjamin A. Pinsky, Evan J. Anderson, Nadine Rouphael
| Format: | Article |
|---|---|
| Diterbitkan: | Centers for Disease Control and Prevention 2020-07-01 |
Deskripsi
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.