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Genetic Diversity Analysis among Greengram genotypes using RAPD Markers
oleh: M.Pandiyan., N.Senthil, P.Sivakumar, AR.Muthiah and N.Ramamoorthi
Format: | Article |
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Diterbitkan: | Indian Society of Plant Breeders 2010-07-01 |
Deskripsi
Green gram is also one of the important pulse crops. Conventional breeding methods are very much difficult to utilize in thedevelopment of new genotypes. Hence incorporation of the molecular approaches along with the conventional techniques ismost powerful method. Evaluation of the available wild accessions are more useful for selecting desirable gene sources.Genetic diversity analysis place an important role in this purpose. For this molecular analysis of selected 18 accessions ingreengram (representing all nine clusters) was carried out through RAPD markers. Out of ten primers used nine werepolymorphic in which the primer OPS-11 exhibited 100 per cent polymorphism. The value of similarity indices 0.72 to 0.91indicates high genetic similarity among the selected accessions at molecular level.